اثر ژل رويال در دوران بارداري تحت استرس بربيان ژن CaMKIIα در ناحيه هيپوكامپ و تست Elevated plus maze در نوزاد رَت نر

Effect of royal jelly during pregnancy under stress condition on hippocampal CaMKIIα gene expression an‎d Elevated plus maze test of male infant rat

زيست شناسي گياهي و جانوري

Prenatal stress is considered one of the key factors affecting the neural an‎d behavioral development of offspring. This study aimed to investigate the effects of royal jelly, as a natural antioxidant supplement, on anxiety-related behaviors, learning performance, an‎d CaMKIIα gene expression in the hippocampus of male rat offspring born to stressed pregnant mothers. In this experimental study, pregnant Wistar rats were divided into four groups: control, stress, royal jelly, an‎d stress + royal jelly. Prenatal stress was induced using a restraint device three times per day from gestational day 7 to 21. Royal jelly was administered orally at a dose of 200 mg/kg to the relevant groups. After 21 days of pregnancy an‎d delivery, the behavior of the offspring was assessed using the Elevated Plus Maze (EPM) an‎d shuttle box test. To eva‎luate molecular changes, CaMKIIα gene expression in the hippocampus was measured using the Realtime PCR technique. The results indicated that prenatal stress increased anxiety-like behaviors, impaired learning, an‎d decreased CaMKIIα gene expression in male offspring. In contrast, maternal royal jelly supplementation during pregnancy reduced anxiety behaviors, improved learning performance, an‎d enhanced CaMKIIα gene expression. These findings suggest a neuroprotective role of royal jelly against the adverse effects of prenatal stress on the neurological an‎d cognitive development of male rat offspring. This may provide a basis for further research into the potential use of royal jelly as an effective intervention for reducing prenatal stress-related complications.

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