تشخيص سريع ملكولي باكتري استرپتوكوكوس پايوژنز با استفاده از روش تكثير هم¬دما

Historically, diseases caused by streptococci have been known for more than two thousan‎d years an‎d remain one of the main causes of health problems in the world today. According to research, streptococci were among the first organisms to be identified as the cause of infectious diseases, an‎d their existence led to the development of sterile methods in their treatment. Streptococci are from the order of lactobacilli an‎d are usually gram-positive, non-motile, facultative anaerobes, an‎d lack the ability to form spores an‎d are oxidase negative. One of the most important species of this family is Streptococcus pyogenes, which is pathogenic. The important point regarding diseases caused by Streptococcus pyogenes is the rapid diagnosis of the pathogen an‎d the initiation of treatment to save the patientʹs life, an‎d most diseases caused by this bacterium are cured by antibiotics. This bacterium produces toxins, including hemolysins, proteases, an‎d superantigens. These toxins cause damage to the kidneys (glomerulonephritis), heart valves (rheumatic fever), skin (scarlet fever), an‎d tissue loss. One of the most common diagnostic methods is to culture the patientʹs saliva an‎d mucus with a swab on agar medium containing blood an‎d a bacitracin disk. If the culture is positive, the appropriate antibiotic for treatment is determined. Considering that the stages of culture an‎d identification of genus an‎d species are time-consuming, the treating physician begins treatment with broad-spectrum antibiotics in the early hours of the onset of clinical an‎d apparent symptoms in suspected an‎d symptomatic patients. These antibiotics have adverse effects an‎d complications. So if the pathogen is identified quickly, antibiotic therapy is directed in a targeted an‎d directed manner. The aim of this study is to identify Streptococcus pyogenes strains by the isothermal loop amplification method. The LAMP test is a fast, cheap an‎d accessible method for detecting an‎d amplifying nucleic acid chains. The primers for this reaction were designed from the specific scpC gene. In the specific identification of the Streptococcus family, the pflB gene was used to design the primer for the isothermal loop amplification technique. Family identification is one of the most recent works carried out in this study an‎d so far there is no research on the specific identification of a family. This study is a small part of a large project in which the aim is to identify an unknown bacterium as quickly as possible. Now, in this study, the specific identification of a part of the Streptococcus family an‎d the identification of the Streptococcus pyogenes bacterium have been addressed.

4

154530