بررسي اثر همافزايي آنتي بيوتيك نايسين با باكتريوفاژهاي اختصاصي باكتريهاي باسيلوس سرئوس و انتروكوكوس فكاليس در شرايط آزمايشكاهي

In vitro investigation of the synergistic effect of the nisin with specific bacteriophages against Bacillus cereus an‎d Enterococcus faecalis.

زيست شناسي سلولي مولكولي و ميكروبيولوژي

Food contamination by pathogenic bacteria is a serious public health concern that can lead to foodborne diseases. Among them, Bacillus cereus, as an opportunistic pathogen, is widely found in a variety of foods. On the other han‎d, Enterococcus faecalis, by forming biofilms, plays an important role in the development of enterococcal infections an‎d resistance to antibiotic treatments, which has presented serious challenges to the treatment of these infections. One of the promising solutions to combact with this problem is the use of biological control by bacteriophages. Nisin, as an antimicrobial protein, is also commonly used in the food industry as an antibacterial agent against Gram-positive bacteria. While bacteriophages have been studied as alternatives to antibiotics, their combination with antibiotics has also shown significant synergistic effects. In this study, the effect of a combination of nisin an‎d specific bacteriophages of Bacillus cereus (bacillus cereus phage CM1) an‎d Enterococcus faecalis (vB_EfaS-SRH2) against Bacillus cereus an‎d Enterococcus faecalis in various foods was investigated, as well as the effect of these two combinations in controlling biofilms of both bacteria an‎d in controlling biofilms of Enterococcus faecalis on urinary catheters, as well as their effect on the survival of zebrafish (Danio rerio). The results showed that the use of the specific bacteriophage combination at MOI=1 along with sub-MIC concentrations of the antibiotic nisin was effective in controlling these pathogens in foods, aquatic environments, an‎d prepared foods. Also, the synergistic effect of phage-nisin completely reduced the biofilm formation potential of Enterococcus faecalis an‎d Bacillus cereus in three conditions: pretreatment, Co- treatment, an‎d posttreatment. Also, the synergy of Enterococcus faecalis-specific bacteriophage (vB_EfaS-SRH2) with nisin showed good performance in controlling an‎d eliminating Enterococcus faecalis biofilm on nelaton an‎d silicone urinary catheters. Also, the synergy of these two compounds showed good performance against Enterococcus faecalis in the treatment of bacteria in the zebrafish (Danio rerio) survival test. Considering the information obtained in this study an‎d also considering the increase in antibiotic resistance an‎d the limitations of the use of antibiotics in food, the combination of nisin with biological control agents such as bacteriophages can be an effective solution for controlling an‎d reducing these microorganisms in food products. This approach not only helps reduce microbial contamination, but can also be considered as a new approach in food safety an‎d the control of bacterial infections.

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