سنجش ميزان بيان lncRNA DAAM2-AS1 در بيماران مالتيپل اسكلروزيس (MS) اصفهان

Multiple sclerosis (MS) is an autoimmune disease of the central nervous system (CNS) characterized by chronic inflammation, demyelination, an‎d loss of neurons. The number of people with MS around the world is reported to be 2.8 million by 2020. Genetic an‎d environmental factors play role in MS, an‎d cells like B an‎d T lymphocytes, macrophages, microglia, astrocytes an‎d etc. are the main players in the pathogenesis of MS, which is usually caused by dysregulation in expression of genes such as cytokines in these cells. This dysregulation is mainly controlled by epigenetic mechanisms especially by lncRNAs. In recent years, several studies have been performed in the field of genetic biomarkers, especially lncRNAs. In the present study, lncRNA DAAM2-AS1 was selec‎ted for investigation using system biology methods an‎d literature review. After getting blood samples from 22 patients an‎d 20 healthy individuals, total RNA extraction from PBMCs, cDNA synthesis an‎d real-time PCR were performed to study the expression of this lncRNA. After statistical analysis by GraphPad Prism software, it was concluded that lncRNA DAAM2-AS1 has no significant expression difference in patients compared to healthy individuals (P Value: 0.626); While theoretically, according to bioinformatics studies, a 10.41-fold increase in expression was reported in the patient group. As a result, the initial hypothesis of a significant increase in expression of this lncRNA in patients compared to the control group was rejected.

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