سنتز نانو كامپوزيت نانوالياف پلي‌كاپرولاكتون و نانوذرات مغناطيسي آهن و كاربرد آن در اندازه گيري ميزان ال-سيستئين

Synthesis of polycaprolactone nanofiber/magnetic nanoparticles nanocomposite an‎d its application in l-cysteine measurements

زيست فناوري

Nowadays, natural enzymes face challenges in industrial an‎d biological applications due to limitations such as low stability an‎d high production costs. As an alternative, nanozymes, particularly nanocomposites based on magnetic iron oxide nanoparticles (Fe₃O₄) with enzyme-like activity, have garnered significant attention. The aim of this study is to synthesize a nanocomposite of polycaprolactone (PCL) nanofibers containing Fe₃O₄ nanoparticles an‎d to investigate its application in the colorimetric detection of L-cysteine. Iron oxide nanoparticles were synthesized via the co-precipitation method. PCL nanofibers were prepared using the electrospinning technique an‎d combined with an optimal concentration of 0.5 wt% Fe₃O₄ nanoparticles. Characterizations using FESEM, EDX, XRD, FTIR, an‎d TGA techniques confirmed uniform distribution of nanoparticles an‎d preservation of PCLʹs semi-crystalline structure. The peroxidase-like activity of the nanocomposite was optimized at pH 3.5 an‎d 40°C, with kinetic parameters Km an‎d for the TMB substrate determined as 1.5 mM an‎d 2.3 × 10⁻⁸ M/s, respectively. The competitive effect of L-cysteine on the peroxidase-like activity was utilized for its detection, achieving a detection limit of 23 µM an‎d a correlation coefficient of R² = 0.992 for the stan‎dard curve an‎d The linear range is 5-40 µM. The nanocomposite demonstrated reusability for several cycles (retaining up to 100% of initial activity after three uses) an‎d holds potential for applications in biosensing an‎d biomedical fields.

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