ارزيابي برون تني زخم‌پوش دولايه پلي كاپرولاكتون - پلي هيدروكسي بوتيرات - كرايسين به روش الكتروريسي و پرينت سه‌بعدي

In vitro eva‎luation of a Bilayer Polycaprolactone/Polyhydroxybutyrate Wound Dressing Loaded with Chrysin, Fabricated by Electrospinning an‎d 3D Printing

مهندسي پزشكي

Introduction: To facilitate optimal wound healing, advanced wound dressings capable of simultaneous therapeutic an‎d protective functions are required. Bilayer dressings offer significant therapeutic potential due to their ability to better mimic the structure of human skin. The objective of this study was the in vitro design an‎d fabrication of an engineered bilayer wound dressing composed of Polycaprolactone (PCL) an‎d Polyhydroxybutyrate (PHB) incorporated with Chrysin, utilizing a combination of 3D printing an‎d electrospinning techniques. Materials an‎d Methods: In this study, the outer layer of the scaffold was fabricated using Polycaprolactone (PCL) via 3D printing. PCL, a biodegradable polyester characterized by high mechanical strength, was utilized to establish a robust structural framework an‎d prevent the ingress of external contaminants. The inner layer comprised Polyhydroxybutyrate (PHB) electrospun nanofibers incorporating various concentrations of Chrysin, deposited onto the printed layer. PHB was selec‎ted as a natural biopolymer due to its superior skin biocompatibility an‎d suitability for drug delivery applications. Chrysin, a natural flavonoid exhibiting antioxidant an‎d anti-inflammatory properties (via inhibition of inflammatory factors such as TNF-α), was loaded into the PHB nanofibers to address its low aqueous solubility an‎d enhance bioavailability. The physicochemical, morphological, an‎d cell adhesion characteristics of the scaffold were characterized using Fourier Transform Infrared Spectroscopy (FTIR) an‎d Scanning Electron Microscopy (SEM). The drug release profile an‎d biocompatibility were assessed via the MTT cytotoxicity assay using fibroblast cell lines. Results: SEM micrographs revealed a bead-free an‎d uniform structure of the electrospun nanofibers. The specimen containing the optimal concentration of Chrysin demonstrated reduced fiber diameter, improved mechanical properties, enhanced hydro‎philicity, an‎d stimulation of cell growth. FTIR analysis confirmed the presence an‎d chemical integrity of all initial components within the final structure. Furthermore, the 3D-printed PCL layer provided the requisite mechanical strength an‎d facilitated the modulation of drug release kinetics from Fickian to non-Fickian diffusion. Conclusion: The findings of this study indicate that the bilayer (PHB-Chrysin)/PCL wound dressing, developed through the integration of 3D printing an‎d electrospinning techniques, exhibits desirable structural properties, controlled drug release, an‎d excellent biocompatibility by leveraging the mechanical strength of PCL an‎d the bioactive, anti-inflammatory attributes of Chrysin-loaded PHB nanofibers. Consequently, this engineered construct emerges as a promising can‎didate for applications in wound healing an‎d skin tissue engineering.

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