چكيده لاتين
Novel wound dressings play a crucial role in effective wound healing. They must maintain optimal moisture, keep water vapor transmission within the therapeutic range, prevent infection, and at the same time, be biocompatible and mechanically stable. The current clinical need is for systems that, in addition to managing exudate and providing physical protection, also offer the controlled release of bioactive agents and microbial inhibition.This research aimed to modify a gelatin-based eutectogel scaffold using decellularized onion and modified silkworm cocoon for use as a wound dressing. Resveratrol, a hydrophobic drug, was selected for the eutectogel system. An examination of the mechanical properties showed that the addition of the decellularized onion composite increased the elastic modulus from 69.56 kPa to 730.17 kPa, and the addition of the modified silkworm cocoon further increased the elastic modulus to 1974 kPa.The evaluation of scaffold swelling confirmed the usefulness of the eutectogel scaffold with decellularized onion, which resulted in a 2.5-fold increase compared to the eutectogel, equivalent to 536.33% ± 4.93. An examination of resveratrol release from the systems showed that in the first 24 hours, the cumulative release percentage was 55.48% ± 2.71 for RES-CEG, 41.84% ± 1.67 for RES-OCEG, and 27.23% ± 1.59 for RES-CCEG. The higher release rate in RES-CEG was consistent with its higher water vapor transmission rate and the hydrophilicity of the matrix. In RES-CCEG, a slower and more controlled release was observed.Biocompatibility (MTT) evaluation showed that the relative cell viability in the RES-CEG, OCEG, and CCEG systems was above 70% on day 1, above 90% on day 3, and above 100% on day 7. Fluorescence images (DAPI) demonstrated a higher cell density in the resveratrol-containing systems, which is consistent with its antioxidant role. The results of the antibiogram test showed that the CEG, OCEG, and CCEG scaffolds were able to create a significant zone of inhibition against Staphylococcus aureus (a Gram-positive bacterium), but no effect was observed against Escherichia coli (a Gram-negative bacterium). Among the samples, only RES-CEG was able to inhibit both bacteria, and its inhibition zone diameter against S. aureus was significantly larger. These findings indicate that the designed scaffolds have higher efficacy against Gram-positive bacteria, and the addition of resveratrol expands their antimicrobial spectrum and enhances their effectiveness.
