ارزيابي قابليت ترميم زخم با استفاده از تركيب سه عنصر: داربست (پرده آمنيوتيك/هيدروژل)، سلول (سلول‌هاي بنيادي و فيبروبلاست) و فاكتور‌هاي محلول (محيط‌كشت شرطي)

eva‎luation of wound healing ability using the combination of three elements: scaffold (amniotic membrane / hydrogel), cell (stem cells an‎d fibroblasts) an‎d soluble factors (conditioned medium)

زيست شناسي گياهي و جانوري

Effective an‎d optimized therapeutic approaches are essential to accelerate the biological regeneration process an‎d prevent chronic complications. Wound healing is a dynamic process involving various cell types, including keratinocytes, fibroblasts, macrophages, an‎d platelets, where any disruption in this system can lead to chronic wound formation. Tissue engineering science, based on three main components—scaffolds, cells, an‎d bioactive molecules—aims to repair damaged an‎d lost tissues an‎d restore their function by utilizing the proliferative an‎d differentiation capabilities of various cells, particularly stem cells, incorporated into diverse biocompatible scaffolds, alongside the application of bioactive molecules. In this study, male Wistar rats were prepared an‎d wounds were induced. The effects of acellular amniotic membrane, hydrogel, fibroblast cells, an‎d conditioned medium on the wound healing process were eva‎luated. Various assessments were conducted, including measurement of wound contraction percentage, epidermal thickness, collagen deposition, an‎d cellular proliferation activity. For this purpose, general hematoxylin-eosin staining, specific Massonʹs trichrome staining, an‎d immunofluorescence methods utilizing the nuclear marker DAPI an‎d antibodies against Ki-67 an‎d Cy5.29 were employed. The results demonstrated that the acellular amniotic membrane group alone had a limited an‎d non-significant effect on wound healing due to the absence of viable cells an‎d active biological factors. However, the combined application of the acellular membrane with hydrogel, hydrogel alone, fibroblasts, an‎d conditioned medium significantly increased wound contraction percentage, epidermal thickness, collagen synthesis, an‎d cellular proliferation activity. The conditioned medium group exhibited the highest collagen accumulation an‎d cellular proliferation activity, highlighting the critical role of growth factors an‎d complex cellular interactions in tissue repair processes. The hydrogel also contributed to enhanced epidermal an‎d extracellular matrix regeneration by providing a three-dimensional hydrated environment an‎d optimal conditions for cell migration an‎d differentiation. Therefore, the findings of this study emphasize that utilizing a combination of supportive biomaterials an‎d viable cells effectively accelerates molecular an‎d cellular mechanisms of wound regeneration an‎d may lead to the development of novel an‎d effective therapeutic strategies for repairing damaged tissues.

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