مطالعه تنوع ژنتيكي جنس.Paeonia L (گل صدتوماني) با استفاده از ماركرهاي مولكولي و داده‌هاي ريز ريخت‌شناسي در ايران

Genetic diversity of the genus Paeonia L. using molecular markers an‎d micro-morphological data in Iran

زيست شناسي گياهي و جانوري

The taxonomy of Paeonia species in Iran is still considered partially unresolved, a condition attributed to the overlap of morphological characteristics an‎d the limited availability of molecular data. In the present study, inter- an‎d intraspecific genetic variation among four taxa, P. archibaldii, P. mascula, P. tomentosa, an‎d P. wendelboi, was assessed using inter-simple sequence repeat (ISSR) markers an‎d micromorphological analyses of leaf epidermal features. A total of 105 ban‎ds were amplified by ten ISSR primers, of which 68 ban‎ds (68.57%) were found to be polymorphic. Cluster analysis an‎d principal component analysis (PCA) identified four main genetic lineages, with P. archibaldii recognized as a relatively distinct sub-lineage nested within the broader P. tomentosa group. Weak geographic structure in populations of P. tomentosa an‎d P. wendelboi was interpreted as indicative of potential gene flow across their northern distribution range. Micromorphological observations using light microscopy an‎d scanning electron microscopy revealed species-specific patterns in stomatal type, density, an‎d cuticular ornamentation, which supported the molecular groupings an‎d facilitated the separation of P. archibaldii an‎d P. mascula. Although P. archibaldii was morphologically distinct, its genetic affinity to P. tomentosa was interpreted as evidence of early divergence rather than complete speciation. The findings demonstrated that the combined application of ISSR marker analysis an‎d micromorphological characters constitutes an effective approach for delimiting species boundaries an‎d resolving taxonomic complexities within the Iranian Paeonia taxa.

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