ساخت، مشخصه‌يابي و ارزيابي هيدروژل پلي‌وينيل‌الكل-سديم‌آلژينات حاوي عصاره گانودرما لوسيدوم براي ترميم زخم

Fabrication, characterization an‎d eva‎luation of Polyvinyl alcohol/ sodium alginate hydrogel containing ganoderma lucidom for wound healing

مهندسي پزشكي

This study aimed at fabrication, characterization an‎d eva‎luation of hydrogel wound dressing for treatment of wound an‎d improve proliferation an‎d regeneration of skin fibroblast cells after any degenerative skin diseases such as diabetic ulcer, burn ulcer, decubitus ulcer, an‎d other physical damages. For this aim, polyvinyl alcohol/ sodium alginate hydrogel was made using freezing/thawing processes. Ganoderma Lucidum extraction was used to improve an‎d accelerate the healing process via maceration method. Then, 5% an‎d 10% amounts (of the polymer solution) were made from the prepared extraction an‎d added to the optimum hydrogel. Morphology of the samples was investigated by SEM, furthermore elongation, maximum tensile strength an‎d youngʹs modulus were eva‎luated an‎d consequently, the final optimum formulation was chosen to incorporate in the Polyvinyl alcohol an‎d sodium alginate hydrogel. Chemical combination of the hydrogels was investigated by FIRE. Next, swelling an‎d stability of the hydrogels were measured. Finally, MTT assay an‎d cells attachment tests were accomplished by L929 cell line after 3 an‎d 7 days cell culture an‎d optimum amount of Ganoderma Lucidum was selec‎ted among two different concentrations 5% an‎d 10%. Moreover, SEM were used to eva‎luate the morphology an‎d attachment of the seeded cell an‎d finally the release rate of the drug loaded on the hydrogels was checked. The results obtained from the optimization of the hydrogel showed that increasing the concentration of SA resulted in a more porous structure an‎d higher elasticity than hydrogel sample Polyvinyl alcohol90%-sodium alginate10%, but decreased the maximum tensile strengths an‎d elongation at break. Adding two 5% an‎d 10% amounts of Ganoderma Lucidum extraction to the optimum hydrogel led to no change in the degradation of the hydrogel substrate, so that the degradation rate of hydrogel sample containing 5% an‎d 10% Ganoderma Lucidum after 28 days is respectively 87% an‎d 83%. The swelling increased with adding 5% an‎d 10% Ganoderma Lucidum extraction in comperative sample without this extraction, an‎d was observed respectively about 164% an‎d 177%. Analysis of drug release indicated appropriate rate of release of Ganoderma Lucidum extraction for hydrogel samples containing 5% an‎d 10% of the extraction for 3 days an‎d the release rate of the extractin for these two samples is respectively 77% an‎d 81%. Furthermore, MTT results represented no cell toxicity effect for the hydrogels without drugs an‎d also the hydrogel samples with the two amounts. Images of the cells seeded on the hydrogel were taken by SEM confirmed suitable attachment of L929 cells on the surface of hydrogel samples, especially at the presence of 5% Ganoderma Lucidum extraction. Polyvinyl alcohol50%-sodium alginate50% hydrogel containing 5% Ganoderma Lucidum extraction could be a promising wound dressing for skin wound healing.

5