بررسي پتانسيل قارچ رشته‌اي نوروسپورا اينترمديا براي توليد تركيب زيست فعال از ويناس

Population growth an‎d decreasing food resources have increased the human need for food alternatives. The increase in waste requires new bioremediation technologies to convert it into valuable materials an‎d protect the environment. Edible filamentous fungi such as Neurospora intermedia produce valuable metabolites such as vitamins, pigments, proteins, an‎d edible biomass by growing on waste such as vinasse. The aim of this study was to investigate the optimal conditions for biomass, carotenoids, an‎d B vitamins production by N. intermedia PTCC 5291, first in basal medium (BMM) with fully controlled conditions an‎d then, our hypothesis is to investigate the effect of optimal BMM conditions on vinasse containing diverse an‎d uncontrollable nutrients. Physiological conditions (including carbon source, then organic an‎d inorganic nitrogen source, carbon to nitrogen ratio, pH, an‎d inoculation percentage), carbon precursors, metal cofactors, an‎d stresses were investigated first in BMM an‎d then in vinasse. The highest biomass yield (8.6 g/L) was obtained by adding olive oil as an additional carbon source. Carotenoid production was increased by the cofactors Mo²⁺ (238.5 μg/g) an‎d ribose (72 h) (203.01 μg/g). The production of B vitamins was increased by the addition of ribose (72 h) an‎d disodium succinate (96 h). The filamentous fungus N. intermedia PTCC 5291 grew well during the bioremediation process according to the results of optimization in vinasse with Brix 6, pH 7, an‎d mineral nitrogen source KNO₃ at 0.5 (g/L). The fungus was able to produce 14.6 g/L of biomass an‎d 90.66 (µg/gDM) of carotenoids. The filamentous fungus biomass was able to reduce COD by 63%. The basal medium produced less biomass than the vinasse medium, an‎d the protein content of the basal medium (17.64 g/100g) was lower than that of the vinasse medium (25.56 g/100g) because the nitrogen content of the BMM medium was lower than that of the vinasse medium. The vinasse medium was more suitable for the growth of N. intermedia than BMM due to its favorable an‎d high levels of carbon, nitrogen, an‎d metals. The metabolic pathway of N. intermedia in the basal medium was more directed towards the production of carotenoids than protein an‎d biomass production compared to the vinasse medium because the high iron content in the vinasse medium inhibited the production of carotenoids. The production of vitamins B1, B2, an‎d B3 was higher in the BMM medium than in the vinasse medium, but there was no significant difference in the production of vitamin B6 in the basal an‎d vinasse media. Our results showed that the filamentous fungus N. intermedia PTCC 5291 is a suitable agent for the production of protein an‎d bioactive compounds such as B vitamins an‎d carotenoids during the second-generation bioremediation process of vinasse. Also, cultivating N. intermedia PTCC 5291 in vinasse reduces the environmental risks of vinasse (melanoidin color an‎d vinasse metals) an‎d makes it a suitable bioremediation food source.

بنياد علم ايران (INSF)

4

دكتر محمد جعفر طاهرزاده

137522