توليد سم زيستي ضد ‌سودوموناس سيرينگه و اروينيا آميلوورا با استفاده از عصاره‌هاي گياهي كپسوله‌شده درون نانوذرات سيليس متخلخل

Organic toxin production against Pseudomonas syringae an‎d Erwinia amylovora using plant extracts encapsulated in mesoporous silica nanoparticles

زيست فناوري

Todays, across the world, the excessive use of chemical pesticides has led to significant environmental difficulties. Therefore, it is crucial to adopt an inexpensive, sustainable, an‎d biocompatible alternative. Natural pesticides have these characteristics, including low cost, availability, biodegradability, an‎d reduced toxicity to living organisms, making them useful alternatives. This study aimed to develop a nano system using mesoporous silica nanoparticles (MSNs) loaded with Zimus Pesticide (ZP) derived from Myrtus communis, Thymus vulgaris, an‎d Curcuma longa, an‎d coated with natural biopolymers Gum Tragacanth (GT) an‎d sodium alginate (SA). Analysis of the extract by HPLC confirmed the presence of phenolic compounds, as the phenol peaks extracted from the extract matched those of the stan‎dard compound. The prepared systems were used to compare antibacterial an‎d antibiofilm effects on Erwinia amylovora an‎d Pseudomonas syringae. The antibacterial activity was measured in CFU/mL for seven days an‎d also by disk diffusion. The MSNs were synthesized an‎d characterized by XRD, SEM, an‎d HR-TEM, exhibiting average pore diameter of 5 nm, the mean particle size is about 40 nm with an amorphous structure. Loading an‎d coating confirmed by TG, TEM an‎d FTIR analysis. ZP was effectively loaded into the MSNs with a loading efficiency of ~25 %, according to TG. TEM images confirmed that a homogeneous coating layer uniformly covered all observed particles. FTIR spectra indicating the successful loading of the extract onto the MSNs an‎d coating of the systems with SA an‎d GT. The GT-MSNs-ZP system showed the highest antibacterial on the seventh day with an inhibitory effect of 42% an‎d 40% an‎d antibiofilm effects with an inhibitory percentage of 49% an‎d 45% at concentrations of 300 an‎d 350 µg/ml, against E. amylovora an‎d P. syringae. GT-MSNs-ZP system released about 56 % of the phenolic compounds over 168 h. Overall, GT is a suitable biopolymer can‎didate for use as the wall material in the formation of encapsulation products, especially plant extracts. These results emphasize the potential of GT-MSNs-ZP as a sustainable an‎d effective biocontrol solution for agricultural applications.

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