طراحي و ساخت داربست زيست‌تخريب‌پذير پلي‌وينيل الكل (PVA)، كيتوسان (CS) و پلي لاكتيك اسيد (PLA) با قابليت رهايش داروي جنتامايسين با استفاده از چاپ چهاربعدي

Design an‎d product of biodegradable polyvinyl alcohol (PVA), chitosan (CS) an‎d polylactic acid (PLA) scaffolds with the ability to release Gentamicin using 4D printing

مهندسي پزشكي

Wound is one of the most common skin injuries that is accompanied by a lot of discharge an‎d infectious problems. For the treatment of wound infection, the use of antibiotics is recommended. Preparing a wound dressing with controlled drug release capability using 4D printing technology is the best measure for local an‎d personalized delivery of antibiotic drugs an‎d reducing the need for oral administration, which will not only have a positive effect on the wound, but also improve an‎d accelerate the wound healing process. In this study, a three-component temperature-sensitive wound dressing with chitosan-polyvinyl alcohol-polylactic acid hydrogel an‎d gentamicin was fabricated using 4D printing technology an‎d its behavior in the presence of glutaraldehyde was studied. The swelling ratio, degradability, water vapor transmission rate, drug release, mechanical properties, surface an‎d morphology of the wound dressing were investigated. The studies conducted showed that the temperature-sensitive wound dressing was successfully prepared. The maximum swelling of the dressing at 49°C was 600% after 600 min an‎d the degradability at the same temperature was 67% after 21 days. The addition of glutaraldehyde to the dressing reduced the degradability an‎d increased the elastic modulus, but reduced its flexibility. Infrared spectroscopy confirmed the interaction of the components. The three-component dressing at 37°C slowly released more than 86% of the initial applied drug over 550 h, confirming the controlled release of the drug. Cytotoxicity using the MTT assay showed cell survival of more than 90% after 7 days, which rules out the possibility of toxicity of the dressing in this study. Antibacterial studies of unmedicated an‎d gentamicin-containing wound dressings showed that gentamicin produced a wide zone of inhibition of growth in three Gram-positive (Staphylococcus aureus) an‎d Gram-negative (Pseudomonas aeruginosa an‎d Escherichia coli) bacteria. The surface roughness of the dressings was high, ensuring their adhesion to the skin. Loading the drug into the dressing increased the roughness by 71%. Changes in the structure of the dressings before an‎d after adding glutaraldehyde an‎d gentamicin were examined with FESEM images, an‎d it was shown that glutaraldehyde improves the structure of the dressing by changing the structure an‎d increasing the pores, an‎d gentamicin also creates protrusions an‎d structural changes, which leads to better adhesion to the wound.

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