توليد ميكربي كاروتنوئيد داينوزانتين توسط سويه هاي استاندارد و جداسازي شده داينوكوكوس رايودورانس با استفاده از سورگوم علوفه اي به عنوان سوبسترا

Microbial production of carotenoid deinoxanthin by stan‎dard an‎d isolated Deinococcus radiodurans strains using forage sorghum as substrate

زيست فناوري

Carotenoids are a large group of compounds composed of repeating isoprenoid units, synthesized by plants an‎d many microorganisms, including algae, bacteria, an‎d fungi. Dinoxanthin is a unique carotenoid extracted from the radiation-resistant bacterium, Deinococcus radiodurans. It is believed that the high resistance of this bacterium to UV-C radiation is due to the protective effect of dinoxanthin present in its cell wall. This pigment possesses antioxidant properties that protect living organisms from reactive oxygen species. Due to these properties, dinoxanthin has been utilized in numerous cancer studies, an‎d its anti-proliferative effects on cancer cells have been observed. In this study, the forage sorghum variety Mansour was used for dinoxanthin production. In the pre-treatment stage, autohydrolysis was considered at temperatures of 120, 150, an‎d 180 degrees Celsius. The Deinococcus radiodurans strain was used for dinoxanthin production. The results indicated that the preferred carbon source for the Deinococcus radiodurans strain was hexose sugars such as glucose. According to the obtained results, the hydrolysate produced during the autohydrolysis stage had a total sugar content ranging from 17.00 to 27.96 grams per liter. By employing an inhibitor removal method using resin an‎d sequential activated carbon methods, liming, an‎d resin were effective in removing phenolic inhibitory compounds by up to 96% for pre-treatment at 120 degrees Celsius for 60 minutes. The highest amount of dinoxanthin produced was 2.5 mg per liter under hydrolysis conditions of 150 degrees Celsius for 60 minutes an‎d diluted five times after inhibitor removal using the sequential activated carbon method, liming, an‎d resin. At 180 degrees Celsius, due to the dark brown color of the environment, the lowest amount of dinoxanthin production was achieved under both inhibitor removal conditions.

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