مطالعه رهايش AGR2 از داربست هيدروژلي و اثر آن بر روي تكثير و مهاجرت سلولي

Study of AGR2 Release from Hydrogel Scaffold an‎d Its Effect on Cell Proliferation an‎d Migration

زيست شناسي

The growth factor Anterior Gradient 2 (AGR2) has been shown to have an effective role in tissue regeneration, but remained largely unexplored in localized tissue engineering applications. Alginate beads have been proven as safe carriers for protein encapsulation, but they suffer from fragility an‎d uncontrolled protein release. For such alginate systems, little is known about how changes in concentrations an‎d ion-crosslinking affect protein release an‎d accumulation in 3-D matrices. To address these questions, an engineered interpenetrating polymer network (IPN) has been used to synthesize a novel hybrid system consisting of AGR2 loaded beads composed of calcium-crosslinked sodium alginate (SA) an‎d carboxymethyl cellulose (CMC). These beads are embedded in films consisting of SA an‎d polyvinyl alcohol (PVA), using a simple ion gelation technique. We assess protein release kinetics an‎d accumulation within the hybrid system by varying polymer concentrations an‎d cross-linking parameters. The IPN hybrid system maintains controlled release over two weeks, without an initial burst period. Through this approach efficient delivery of AGR2 is achieved which in turn effectively mediates cell migration an‎d proliferation, resulting in excellent cell viability an‎d complete wound closure. The described release system opens new perspectives in tissue engineering. Keywords: Alginate beads; Anterior gradient 2 (AGR2) growth factor; Interpenetrating polymer network (IPN); Tissue engineering.

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