تهيه، شناسايي و كاربرد نانوذرات پلي‌پايرول توخالي براي رهاسازي متوتركسات با ولتاژ

Preparation, characterization an‎d application of hollow polypyrrole nanoparticles for voltage triggered methotrexate release

شيمي تجزيه

Despite significant advancements in cancer treatment research, conventional methods, including chemotherapy, remain the primary options. The main disadvantage of these treatments, besides the low solubility of many antitumor drugs, is their lack of specificity. This lack of specificity leads to severe side effects, due to the non-specific uptake of the drug into healthy cells. Advances in drug delivery systems can reduce side effects, increase the circulating half-life, an‎d improve pharmacokinetics. In this study, the biocompatible, biodegradable an‎d pH-responsive hydroxyapatite nanosystem was prepared. Then, the polypyrrole coating was applied around the nanoparticles, an‎d the hydroxyapatite nanoparticles were removed. The anticancer drug methotrexate was loaded into the remaining cavities of the polymer. This system was characterized by various methods. The biocompatibility of nanocarriers was measured using the MTT assay, an‎d their anticancer efficiency was eva‎luated by studying of cell apoptosis, cell cycle, an‎d cell uptake assay. The hydrodynamic size of the nanocarriers was 214 nm. Drug release from the synthesized nanocarriers was performed by applying of direct contact electrical potential (voltage stimulation method), an‎d the amount of released drug was determined by UV-Vis spectrophotometry. The optimal ratio for drug loading was found to be 0.85 mg of drug per mg of the nanosystem. For the amount of loaded drug, the values of LD an‎d LE were calculated to be 42% an‎d 90%, respectively.

3

122200