بررسي بيان RNA بلند غير رمز گردان LINC01117 در سرطان پستان

Breast cancer is a type of malignant tumor that has been reported as the most common cancer worldwide in recent years. Breast cancer is a highly heterogeneous disorder, with variations in its causes, early symptoms, treatment methods, and treatment response from one person to another. Although significant progress has been made in implementing personalized treatment strategies for breast cancer, therapeutic outcomes are often unsatisfactory due to the late diagnosis of the disease. Various studies have shown that long non-coding RNAs (LncRNAs) exhibit abnormal expression in breast cancer and are closely associated with tumor progression and development. Therefore, identifying differences in gene expression between normal and tumor tissues is of high clinical importance for the early detection of the disease. The aim of this study is to investigate the expression levels of the long non-coding RNA LINC01117 in tumor tissue samples compared to normal adjacent breast tissue, which has not yet been explored in laboratory studies. In this study, 386 tumor and normal adjacent breast tissue samples were collected. After excluding samples with incomplete clinical pathology data, non-Luminal B samples, and those with insufficient RNA concentration and purity, 62 samples were selected for single-strand cDNA synthesis and gene expression analysis using Real-time PCR. Statistical analyses showed a significant increase in expression in tumor tissue samples compared to normal adjacent tissue samples, with a P-value of 0.001. The diagnostic potential of LINC01117 gene expression was also assessed through an ROC curve analysis, revealing that LINC01117 gene expression could serve as a potential early diagnostic marker for breast cancer. Additionally, bioinformatics and systematic studies were conducted to eva‎luate the prognostic impact of high expression of this gene. These studies indicated that patients with elevated LINC01117 expression have a poorer prognosis, are more likely to experience recurrence or metastasis, and have lower survival rates compared to patients with lower expression of this gene.

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