تهيه جاذب كامپوزيت پليمري پلي اتيلن ايمين و پلي وينيل الكل براي استخراج و اندازه‌گيري دارو‌هاي ضد التهاب غير استروئيدي

In this research, iron oxide magnetic nanoparticles coated with PVA/PEI composite were synthesised and used as a sorbent for extraction and preconcentration non-steroidal anti-inflammatory drugs (NSAIDs). High-performance liquid chromatography (HPLC) equipped with a photodiode array detector was used to determine the amount of NSAIDs. Factors affecting the synthesis of coated magnetic nanoparticles, including the weights of iron (ɪɪ) and iron (ɪɪɪ), ratio of PVA to PEI, volume of glutaraldehyde (GLA), mixing time of PVA with PEI and the crosslinking time were investigated. The weights of iron (ɪɪ) and iron (ɪɪɪ) were 0.043 and 0.106 g and the ratio of PVA to PEI was 2 to 1. The volume of GLA, the time of mixing PVA with PEI and the time required to establish the crosslinker were selected as 60µL, 90 and 60 minutes, respectively. Various methods were used to characterize coated magnetic nanoparticles, including Fourier transform infrared spectroscopy, alternating gradient force magnetometry and transmission electron microscopy. Also, the effective factors on the extraction of drugs were studied and the optimal condition obtained which are: 50 mg of absorbent (iron oxide nanoparticles with PVA/PEI coating), pH of the sample solution equal to 2.0,1 minute of absorption time, 400 µL of acetonitrile as desorption solvent and 1 minute time required for desorption. The calibration curves of each of the drugs were drawn in optimal conditions and based on these curves, the figures of merit, including the working linear range (50.0 -1600.0 μg/L) with R2 >0.997, presicion(RSD<2.69%), limit of detection (35.6, 35.6 and 29.8 μg/L) and accuracy (recovery>89%) were eva‎luated for Ketorolac, Naproxen and Diclofenac respectively. Finaly the proposed method was used for extracting and determining the amount of NSAIDs in a groundwater sample.

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