بررسي اثر سفيده ي تخم هشت گونه پرنده بر روي باكتري هاي بيماري زا و لنفوسيت هاي انسان و مطالعه ي ژن هاي پروتئين اووالبومين موجود در سفيده با استفاده از روش¬هاي بيوانفورماتيكي

Avian albumen is a complex multifunctional medium promoting the growth of the embryo.It is composed of various important nutrients such as proteins, lipids, enzymes and various biologically active substances including growth promoting factors. Avian egg whites contain many proteins that have specific properties such as Ovotransferrin (Conalbumin), Ovalbumin, Lysozyme, Ovomucin, Ovomucoid, Ovoglobulin, Avidin, Cystatin, Ovoflavoprotein and Ovoinhibitor. In recent decades, several studies have been carried out about anti-bacterial, anti-viral, anti-cancer and anti-inflammatory activities of egg whites. The aims of this study were to eva‎luate antibacterial and cytotoxicity effects of eight bird egg whites against 10 pathogenic bacteria and human peripheral blood mononuclear cells (PBMCs). The studied species were Meleagris gallopavo, Bambusicola thoracicus, Columba livia , Gallus gallus, domestic Gallus gallus, Coturnix coturnix, Anas platyrhynchos and Melopsittacus undulatus. The antibacterial and cytotoxic activities of different concentration of egg whites were performed by disk diffusion and MTT assays respectively. Also, in the present study, we have developed a computer based method for the classification and screening of ovalbumin genes based on the pseudo nucleotide composition (PseKNC) concept and machine learning algorithms. For this propose, different algorithms were compared based on their performance in classifying and predicting ovalbumin genes. The results showed that the Neural net algorithm had the best performance with accuracy of 98.8 percent in predicting ovalbumin genes. The results of MTT assay showed that All these eggs white increased lymphocyte cell proliferation. The most and the least active eggs white on PBMC proliferation were related to Anas platyrhynchos and Melopsittacus undulates respectively. The result of antibacterial assay demonstrated that all egg whites have an antibacterial effect against bacteria except Melopsittacus undulates. The highest and lowest anti-bacterial effects were obtained in the presence of domestic Gallus gallus and Columba livia eggs white respectively.

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