بررسي و طبقه بندي داده‌هاي مرتبط با كاربرد دِنازيم‌هاي تنظيم‌كننده‌ي بيان ژن

eva‎luation and classification of the data on the application of gene-regulating DNAzymes

زيست شناسي سلولي مولكولي و ميكروبيولوژي

DNAzymes are single-stranded oligodeoxynucleotides with catalytic properties, which have been discovered for about three decades, and so far more than 1500 different DNAzyme sequences have been designed and isolated, which are capable of catalyzing at least 20 different reactions. In the meantime, the most important reaction catalyzed by this group of biocatalysts is the RNA cleavage reaction, which gives DNAzymes the ability to silence the expression of the target gene, relying on the target messenger RNA cleaving, and this class of functional nucleic acids has changed into a powerful tool in the field of the diagnosis and treatment of diseases. Despite the high importance of the field of gene expression regulation by DNAzymes and the multitude of important indicators surrounding the use of gene expression silencing DNAzymes, until now, there is no online database related to the mentioned field. Therefore, the current research eva‎luates and classifies the data related to the studies conducted in the field of gene expression regulation by DNAzymes to finally provide the basis for the design and development of an online database based on the aforementioned classified data. In this regard, 96 research articles and references related to the use of DNAzymes against 12 different target carcinogenic genes were selected and the desired data were extracted from them and finally classified. In parallel, the bodyʹs basic shape and the way the database works were drawn and compiled. It should be noted that the DNAzymeExplorer database has an information domain related to 41 different DNAzymes, as well as 917 laboratory and biological assays conducted in line with the application and reading of the activity related to the desired DNAnzymes, against the target genes. The DNAzymeExporer database will have a user-friendly network interface and an open application programming interface, which will allow the user and researcher to easily and quickly search, compare, and store the sequence of DNAzymes and their surrounding information, as well as information related to the applied assays and readout of DNAzyme activity.

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