گلايكيشن سلولهاي فيبروبلاست پوست و مهار توسط كوئرستين

In this research, the viability of human dermal fibroblast (HDF) cells was eva‎luated in vitro and in hyperglycemic conditions. In order to mimic hyperglycemic conditions, fructose glycated bovine serum albumin (BSA) was used. Then, the destructive effect of AEGs obtained from fructosylated BSA on the survival of HDF was studied. In the next step, the effectiveness of quercetin in protecting HDF against the mentioned hyperglycemic conditions, cell viability, and apoptosis were measured. MTT test, inverted microscope images, and ANNEXIN-V FITC kit were used to accurately examine cell viability, apoptosis, and necrosis. The results showed that AEGs decrease the viability depending on the concentration and glucose-AEGs cause cell death more strongly. Treatment of cells with quercetin after exposure to AEGs, glucose, and glucose-AEGs showed that concentrations of 25 μM and 50 μM quercetin significantly reduced cell death, while 10 μM concentration had no significant effect on increasing cell viability. The formation process of AGEs was eva‎luated by absorbance methods, intrinsic fluorescence, thioflavin T (THT) fluorescence, and fluorescence microscopy. Quercetin was effective in reducing cell death with a large difference compared to the control sample. The obtained findings can pave the way for the design of new drugs to control the complications caused by hyperglycemia and diabetes.

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