ارزيابي نقش جينجرنونA در توليد راديكال‌هاي آزاد اكسيژن در رده‌ي سلولي لوسمي لنفوبلاستي حاد كودكان

eva‎luation of The Role of Gingerenone A In The Production of Oxygen Free Radicals In The Pediatric Acute Lymphoblastic Leukemia Cell Line

زيست شناسي سلولي مولكولي و ميكروبيولوژي

Acute lymphoblastic leukemia (ALL) is an aggressive blood malignancy that is caused by the proliferation and spread of immature lymphoid progenitor cells in the blood, bone marrow, and other organs. It is the most common type of cancer in children. The T-type immunophenotype (T-ALL) is less common but more invasive than type B. Gingerenone A is one of the derivatives of the ginger medicinal plant, which our group has proven its anti-leukemic effects. This research aimed to investigate the effect of gingerenone A on the T-type malignant cell line, CCRF-CEM, considering the possibility of inducing oxygen free radicals in these cells. After treatment with concentrations of 10 and 40 μM of gingerenone A, T cells died by 43.33% and 23.33%, respectively, compared to the control group. Also, the results of flow cytometry indicated that the mentioned concentrations caused an increase of cellular free oxygen radicals 5.233 and 7.298 times more than the control group, respectively. Data obtained following the treatment of the gingerenone A-treated CCRF-CEM cells with N-acetylcysteine, a free radical inhibitor, were reversed. In addition, the flow cytometry data examining the DNA content in cells treated with the aforementioned concentrations of gingerenone A showed that following the increase of free radicals, the cell cycle arrests at the SubG1 phase. This research is a step towards identifying the cytotoxic and cytostatic effect of gingerenone A on a T-ALL cell line, potentially contributing to the development of new treatment strategies for this disease.

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