چكيده لاتين
The aim of the current research is to characterize the temperature-sensitive injectable hydrogel for the engineering of damaged heart tissue after myocardial infarction. For this purpose, hydrogel sensitive to Pluronic temperature, chitosan and β-glycerophosphate as chitosan cross-linking agent and gold nanocolloid as electrical conductivity agent for heart tissue were made. Also, polyglycerol sebasate polymer, which was synthesized by a multi-step reaction method, was used as a carrier of omega-3 drugs. By solvent replacement method, six formulations of polyglycerol sebasate nanoparticles containing omega-3 were prepared. The optimal formulation was selected based on particle size, zeta potential, and dispersion index and was used to check the drug loading and release rate. The time required for the gelation process of the hydrogel at 37 ℃, its stability and injectability were measured. Its rheological property was observed by rheometer, and its chemical composition was observed by FTIR. Also, the electrical conductivity of the hydrogel was evaluated by a conductometer. The optimal nanoparticle contained 5% w/v polyglycerol sebasate and a ratio of one to five organic phase to aqueous phase. The particle size of the optimal formulation was 167.6 nm, zeta potential mv -25, dispersion index was 0.124, drug loading rate was 89.7% and release after one month was 20.4%. Injectable temperature-sensitive hydrogel with 15% Pluronic, 1% chitosan, 16% β-glycerophosphate and 1% v/v gold Nano- colloid and polyglycerol sebasate nanoparticles was selected as the composition used. Gold nanoparticles have an electrical conductivity of 1400 μS/cm created for hydrogel. The research showed that the selective polymer and polyglycerol sebasate nanoparticles containing omega-3 are not a suitable option for the application of heart tissue engineering in ischemic conditions due to the slowly release rate and low drug release rate. Also, Due to the hydrophobic nature of omega-3, it is not possible to load the drug into the hydrogel.
