تعيين اثرتمرين هاي مقاومتي، استقامتي و تزريق ايريزين بر بيان miR-223a، ژن و پروتئين HSP-70 در كبد موش هاي سوري

eva‎luation of the effect of resistance and endurance training and irisin injection on miR-223a and HSP-70 gene and protein in mice liver

فيزيولوژي ورزش

Background: Physical inactivity and aging are associated with many metabolic complications, especially due to oxidative stress of the liver. The aim of this study was to determine the effect of resistance, endurance exercises and irisin injection on the expression of miR-223a, HSP-70 gene and protein in the liver of Syrian mice. Methods: In this project, 28 NMRI strains of mice prepared from Enistito Pasteur of Iran were tested. The tested animals were kept in transparent polycarbonate cages in groups of 5 rats during a two-week period of adaptation to the new environment and familiarization with the conveyor belt as well as the implementation of the protocol. The mice were randomly divided into 4 groups after two weeks of familiarization with the laboratory environment, and the number of mice in each group was 7. It included the control group, endurance training group, resistance training group and Ayrazine injection group. Endurance training was done by running on a treadmill. In the first and second week, the treadmill was set at a zero degree angle and a speed of 5 meters per minute. This amount gradually increased and reached a slope of 5 degrees and a speed of 15 meters per minute. This work was done in 4 sets of 5 repetitions with 2 minutes of rest between sets for 8 weeks and 3 sessions per week. Resistance exercises were performed using a ladder. Training started with 30% of body weight in the first week and reached 200% of body weight in the eighth week. Ayrazine was also injected intraperitoneally, 3 days a week, with a dose of 100 mg-1 kg per week. After the completion of Ayrezin's training period, miR-223a and HSP70 gene expression were measured by real-time PCR and HSP-70 protein expression by Western blot method. Statistical analysis was done through one-way analysis of variance with Tukey's post hoc test at a significance level of 0.05 by SPSS version 26 software. Results: There is a significant difference between aerobic training, resistance training and Ayrazine injection on miR-223a expression, HSP-70 gene expression, HSP-70 and Ayrazine protein levels in the liver of Syrian mice (P<0.05). Changes in miR-223a decreased, HSP-70 gene expression and HSP-70 and Ayrsin protein levels increased. Conclusion: Based on the results of the present study, both resistance and endurance exercises can affect miR-223a expression, HSP-70 gene expression, HSP-70 protein levels and ayrazine in the liver of Syrian mice. Sports activity seems to have beneficial effects in the prevention and treatment of metabolic diseases through these changes.

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سعيد زماني ، محمدرضاشريفي