چكيده لاتين
Prediabetes is a risk factor for metabolic syndrome and people with prediabetes may eventually develop diabetes. Therefore, promoting a healthy lifestyle is essentinal to prevent prediabetes. This study was conducted with the aim of determining the effect of green coffee, chlorogenic acid and exercise training on lipid metabolism in the liver of prediabetic C57BL/6 male mice. In the first phase of the study for 12 weeks, forty-nine male C57BL/6 mice were randomly divided into two groups and given a normal diet (ND, n = 7) or a high-fat diet (HFD); Then, in the second phase of the study, prediabetic (preD) mice were divided into six groups (n=7): 1) preD, 2) green coffee (GC), 3) chlorogenic acid (CGA), 4) exercise (EX) 5) GC+EX, and 6) CGA+EX. Treadmill exercise was performed for 45 minutes per day, 5 days per week for 10 weeks, and GC and CGA gavage continued for 10 weeks along with HFD. Plasma and liver samples were prepared at the end of the second stage and biochemical analysis, RT-PCR and immunoblotting were performed. The results obtained in this study indicate that prediabetes caused by HFD can be improved with endurance training and GC and CGA supplementation, as well as the simultaneous application of interventions. Among these findings, fast glucose level, insulin, and HOMA indicated a significant increase in prediabetes compared to ND and decrease significantly with the application of all interventions. lipid profile of sampls, reveals a significant increase in the preD group and a significant decrease in other groups (except EX in the HDL). ALT and AST, which were increased due to HFD consumption in the plasma of the preD group, decreased with the application of treatments in most of the intervention groups. The increase in acyl-CoA production due to ACSL3 in preD mice was reversed by exercise training and GC and CGA supplementation and their simultaneous application. Also, DNL, which is known by ACC1, Fasn and Srebp1c, was increased in preD, which decreased with exercise training and GC and CGA either alone or at the same time. The increase in TG production in preD through GPAT1 was also reduced by exercise training and GC and CGA supplementation and simultaneous interventions. Also, the results of the study show that the increase in lipolysis, which is known by the function of CPT1 and Pparα, as well as the decrease in β-oxidation due to the effect of ACC2 in preD, were improved by exercise training and GC and CGA supplementation and simultaneous induction of treatments. These findings show that regular exercise is an effective non-therapeutic approach for people with prediabetes and CGA and GC supplementation can be an alternative to mimic some of the beneficial effects of exercise on prediabetes.
