بررسي بيان ژن FOXE1 در نئوپلاسم تيروئيد در جمعيت اصفهان

eva‎luation of FOXE1 gene expression in thyroid neoplasm in Isfahan population

زيست شناسي سلولي مولكولي و ميكروبيولوژي

Thyroid neoplasm is the most common endocrine cancer and has a growing rate in the world and in Iran. FOXE1 gene (NCBI_ID = 2304) is one of the most important genetic factors related to the growth and development of the thyroid gland and is responsible for the proliferation and differentiation of thyroid follicular cells. In adults, this gene plays an essential role in controlling thyroid hormone synthesis, regulating thyroglobin, and expressing the thyroid peroxidase gene. In this study, the expression of FOXE1 gene in 21 samples, including 11 tumor samples and 10 control samples was eva‎luated by RT-qPCR technique. Data were statistically analyzed using REST 2009 software. Healthy specimens adjacent to the tumor with a nodule size less than 20 mm were compared with healthy specimens adjacent to the tumor larger than 20 mm, and a significant increase in FOXE1 gene expression (p-value =0.039) was observed. Also, FOXE1 mRNA expression in small nodules with metastasis showed a 27.858 increase in expression compared to small nodules without metastasis (p-value < 0.001). The expression level of FOXE1 gene in tumor samples showed a slight decrease in expression and this decrease in expression was not statistically confirmed (p-vlue = 0.918). The relationship between increased FOXE1 mRNA expression with increasing age (over 40 years) and calcification was investigated but not statistically confirmed. In conclusion, FOXE1 gene expression showed a direct relationship with nodule size and metastasis in patients in Isfahan population.

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