5928

methods and protocols

edited by Jun Zhang and Gregg Rokosh[electronic resource]

Humana Press

c2007

xi, 365 p. ill. 24 cm

Methods in molecular biology

Includes bibliographical references and index , Cardiac gene expression: the global perspective. Microarray analysis of gene expression in murine cardiac graft infiltrating cells / Yurong Liang, Xin Lu, and David L. Perkins. Expression profiling using Affymetrix GeneChip probe arrays / Martina Schinke-Braun and Jennifer A. Couget. Serial analysis of gene expression (SAGE): a useful tool to analyze the cardiac transcriptome / Kirill V. Tarasov ... [et al.] Functional genomics by cDNA subtractive hybridization / Christophe Depre. Statistical methods in cardiac gene expression profiling: from image to function / Sek Won Kong -- Cardiac gene regulation: gene-specific mRNA measurement in the myocardium. Measurement of cardiac gene expression by reverse transcription polymerase chain reaction (RT-PCR) / Nicola King. Quantitative (real-time) RT-PCR in cardiovascular research / Kevin John Ashton and John Patrick Headrick. RNase protection assay for quantifying gene expression levels / Yongxia Qu and Mohamed Boutjdir. In situ hybridization: a technique to study localization of cardiac gene expression / Thierry P. Calmels and David Mazurais -- Cardiac gene regulation: promoter characterization in the myocardium. Characterization of cis-regulatory elements and transcription factor binding: gel mobility shift assay / Jim Jung-Ching Lin ... [et al.] -- Mapping transcriptional start sites and in silico DNA footprinting / Martin E. Cullen and Paul J.R. Barton. Characterization of cardiac gene promoter activity: reporter constructs and heterologous promoter studies / Hsiao-Huei Chen and Alexandre F.R. Stewart -- In silico assessment of regulatory cis-elements and gene regulation. Comparative genomics: a tool to functionally annotate human DNA / Jan-Fang Cheng, James R. Priest, and Len A. Pennacchio. Developing computational resources in cardiac gene expression / Michael B. Bober and Raimond Winslow -- Cardiac single nucleotide polymorphisms. In silico analysis of SNPs and other high-throughput data / Neema Jamshidi, Thuy D. Vo, and Bernhard O. Palsson. Discovery and identification of sequence polymorphisms and mutations with MALDI-TOF MS / Dirk van den Boom and Mathias Ehrich -- Gene overexpression and targeting in the myocardium. Conditional targeting: inducible deletion by cre recombinase / Kelly R. OʹNeal and Ramtin Agah. Cardiomyocyte preparation, culture, and gene transfer / Alexander H. Maass and Massimo Buvoli. Adeno-associated viral vector-delivered hypoxia-inducible gene expression in ischemic hearts / Hua Su and Yuet Wai Kan. Lentivirus-mediated gene expression / Jing Zhao and Andrew M.L. Lever. , Mode of access: World Wide Web

Rokosh, Gregg. , Zhang, Jun,