5918

methods and protocols

edited by Clare Davy, John Doorbar

Humana Press

c2005

xv, 496 p. ill. 24 cm

Methods in molecular medicine

Includes bibliographical references and index , Identification of new papillomavirus types / Ethel-Michele de Villiers, Corinna Whitley, and Karin Gunst -- Identification of HPV variants / John Cason, Jon Bible, and Christine Mant -- Histochemical analysis of cutaneous HPV-associated lesions / Kiyofumi Egawa -- Histochemical analysis of cervical intraepithelial neoplasia / Michael Babawale ... [et al.] -- Detection of papillomavirus proteins and DNA in paraffin-embedded tissue sections / Woei Ling Peh and John Doorbar -- Detection and quantitation of HPV gene expression using real-time PCR / Rashmi Seth ... [et al.] -- Analysis of P16INK4A and integrated HPV genomes as progression markers / Svetlana Vinokurova, Nicolas Wentzensen, and Magnus von Knebel Doeberitz -- Use of biomarkers in the evaluation of CIN grade and progression of early CIN / Jan P.A. Baak and Arnold-Jan Kruse -- HPV DNA detection and typing in cervical scrapes / Peter J. F. Snijders ... [et al.] -- HPV DNA detection and typing in inapparent cutaneous infections and premalignant lesions / Maurits de Koning ... [et al.] -- Establishing HPV-containing keratinocyte cell lines from tissue biopsies / Margaret Anne Stanley -- Using an immortalized cell line to study the HPV lifecycle in organotypic "raft" cultures / Paul F. Lambert ... [et al.] -- , Differentiation of HPV-containing cells using organotypic "raft" culture or methylcellulose / Regina Wilson and Laimonis A. Laimins -- Propagation of infectious, high-risk HPV in organotypic "raft" culture / Margaret E. McLaughlin-Drubin and Craig Meyers -- Retrovirus-mediated gene transfer to analyze HPV gene regulation and protein functions in organotypic "raft" cultures / N. Sanjib Banerjee, Louise T. Chow, and Thomas R. Broker -- The HPV xenograft severe combined immunodeficiency (scid) mouse model / William Bonner -- The cottontail rabbit papillomavirus model of high-risk HPV-induced disease / Janet L. Brandsma -- Studying the HPV lifecycle in 3A trophoblasts and resulting pathophysiology / Yong Liu, Hong You, and Paul L. Hermonat -- Replication and encapsidation of papillomaviruses in saccharomyces cerevisiae / Peter C. Angeletti -- Analysis of the regulation of viral transcription / Bernd Gloss, Mina Kalantari, and Hans-Ulrich Bernard -- Analysis of HPV transcripts by RPA / Jason M. Bodily and Craig Meyers -- Analysis of regulatory motifs within HPV transcripts / Sarah A. Cumming and Sheila V. Graham -- Detection of HPV transcripts by nested RT-PCR / Christine Mant, Barbara Kell, and John Cason -- , Analysis of HPV DNA replication using transient transfection and cell-free assays / Bing Yuan Lin, Thomas R. Broker, and Louise T. Chow -- Detection and quantitation of HPV DNA replication by southern blotting and real-time PCR / Iain M. Morgan and Ewan R. Taylor -- Analysis of E7/RB associations / Sandra Caldeira, Wen Dong, and Massimo Tommasino -- Transformation assays for HPV oncoproteins / Paola Massimi and Lawrence Banks -- Analysis of adeno-associated virus and hpv interaction / Paul L. Hermonat ... [et al.] -- In vitro assays of substrate degradation induced by high-risk HPV E6 oncoproteins / Miranda Thomas and Lawrence Banks -- Measuring the induction or inhibition of apoptosis by HPV proteins / Anna M. Kowalczyk ... [et al.] -- Codon optimization of papillomavirus genes / Martin Muller -- Generation of HPV pseudovirions using transfection and their use in neutralization assays / Christopher B. Buck ... [et al.] -- Generation and application of HPV pseudovirions using vaccinia virus / Martin Sapp and Hans-Christoph Selinka.

Davy, Clare. , Doorbar, John.

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