5910

methods and protocols

edited by Alexander E. Kalyuzhny.[electronic resource]

Humana Press

c2005

xiv, 321 p. ill. 24 cm

Methods in molecular biology

Includes bibliographical references and index , The ELISPOT assay : a personal retrospective / Jonathan Sedgwick -- Chemistry and biology of the ELISPOT assay / Alexander E. Kalyuzhny -- Membranes and membrane plates used in ELISPOT / Alan J. Weiss -- Standardization and validation issues of the ELISPOT assay / Sylvia Janetzki ... [et al.] -- A cell detachment solution can reduce a background staining in ELISPOT assay : Accumax / Angela Grant ... [et al.] -- Isolation of subsets of immune cells / Carrie E. Peters, Steven M. Woodside, and Allen C. Eaves -- Image analysis and data management of ELISPOT assay results / Paul Viktor Lehmann -- High resolution as a key feature to perform accurate ELISPOT measurements using Zeiss KS ELISPOT readers / Wolf Malkusch -- , Improving the sensitivity of the ELISPOT analyses of antigen-specific cellular immune responses in rhesus macaques / K. Jagannadha Sastry, Pramod N. Nehete, and Bharti Nehete -- Feline cytokine ELISPOT : issues in assay development / Sushila K. Nordone ... [et al.] -- Measuring T cell function in animal models of tuberculosis by ELISPOT / Vanja Lazarevic, Santosh Pawar, and JoAnne Flynn -- IFN-gamma ELISPOT assay for the quantitative measurement of antigen-specific murine CD8+ T cells / Geoffrey A. Cole -- Detection of measles virus-specific IFN-gamma secreting T cells by ELISPOT / Jenna E. Ryan, Inna G. Ovsyannikova, and Gregory A. Poland -- Epitope mapping in multiple sclerosis using the ELISPOT assay / Clara M. Pelfrey and Ioana R. Moldovan -- , Use of IFN-gamma ELISPOT in monitoring immune responses in humans / Mark Matijevic and Robert G. Urban -- ELISPOT determination of IFN-gamma T cell frequencies in patients with autoimmune sensorineural hearing loss / C. Arturo Solares and Vincent K. Tuohy -- Dual color ELISPOT assay for analyzing cytokine balance / Yoshihiro Okamoto and Mikio Nishida -- Simultaneous detection of multiple cytokines in ELISPOT assays / Sarah Palzer ... [et al.] -- Fluorospot assay : methodological analysis / Agnes Gazagne ... [et al.] -- A gel-based dual antibody capture and detection method for assaying of extracellular cytokine secretion : EliCell / Lisa A. Spencer ... [et al.] , Mode of access: World Wide Web

Kalyuzhny, Alexander E