5902

methods and applications

edited by Piet Herdewijn

Humana Press

c2005

xvi, 435 p. ill. 24 cm

Methods in molecular biology

Includes bibliographical references and index , Synthesis of DNA using a new two-step cycle / Douglas J. Dellinger ... [et al.] -- RNA synthesis using 2ʹ-O-(tert-butyldimethylsilyl) protection / Brian S. Sproat -- RNA oligonucleotide synthesis via 5ʹ-silyl-2ʹ-orthoester chemistry / Stephanie A. Hartsel... [et al.] -- Dimethylthiarum disulfide: new sulfur transfer reagent in phosphorothioates oligonucleotide synthesis -- Zhiwei Wang, Quanlai Song, and Yogesh S. Sanghvi -- Assay for evaluating ribonuclease H-mediated degradation of RNA-antisense oligonucleotide duplexes / Annie Galarneau ... [et al.] -- Di- and oligonucleotide synthesis using H-phosphonate chemistry / Jacek Stawinski and Roger Str�omberg -- Solid-phase synthesis of circular oligonucleotides / Enrique Pedroso ... [et al.] -- Locked nucleic acid synthesis / Henrik M. Pfundheller ... [et al.] -- Synthesis of DNA mimics representing HypNa-pPNA hetero-oligomers / Vladimir A. Efimov and Oksana G. Chakhmakhcheva -- Base-modified oligonucleotides with increased duplex stability: pyrazolo[3,4-d]pyrimidines replacing purines / Frank Seela ... [et al.] -- Introduction of hypermodified nucleotides in RNA / Darell R. Davis and Ashok C. Bajji -- Chemical methods for peptide-oligonucleotide conjugate synthesis / Dmitry A. Stetsenko and Michael J. Gait -- Biotin-labeled oligonucleotides with extraordinarily long tethering arms / A. Michael Morocho ... [et al.] -- Universal labeling chemistry for nucleic acid detection on DNA chips -- Ali Laayoun ... [et al.] -- Postsynthetic functionalization of triple helix-forming oligonucleotides / Alexandre S. Boutorine, and Jian-Sheng Sun -- Fluorescence-based On-line detection as an analytical tool in RNA electrophoresis / Ute Scheffer and Michael G�obel -- Design and optimization of molecular beacon real-time polymerase chain reaction assays / Jacqueline A.M. Vet and Salvatore A.E. Marras -- High-thoughput production of optimized primers (fimers) for whole-genome direct sequencing / Nikolai Polushin ... [et al.] -- Nonenzymatic template-directed RNA synthesis / Marcus Hey and Michael G�obel -- DNAse I footprinting of small molecule binding sites on DNA / Christian Bailly ... [et al.] -- Gene targeting using peptide nucleic acid / Peter E. Nielsen -- Nucleic acid library construction using synthetic DNA constructs / Hani S. Zaher and Peter J. Unrau -- In vitro selection from combinatorial nucleic acid libraries / Andres J�aschke -- In vitro selection procedures for identifying DNA and RNA aptamers targeted to nucleic acids and proteins / Eric Dausse ... [et al.] -- Short double-stranded ribonucleic acid as inhibitor of gene expression by the interference mechanism / Jean-R�emi Bertrand, Andrei Maksimenko, and Claude Malvy.

Herdewijn, Piet