5526

cyclins, cyclin-dependent kinases, and other factors

edited by Antonio Giordano, Gaetano Romano

Humana Press

c2004

xiii, 185 p. ill. (some col.) 23 cm

Methods in molecular biology

Includes bibliographical references and index , The biology of cyclins and cyclin-dependent protein kinases: an introduction / Lucio Miele -- In situ immunofluorescence analysis: immunofluorescence microscopy / Amjad Javed ... [et al.] -- In situ immunofluorescence analysis: analyzing RNA synthesis by 5-bromouridine-5ʹ-triphosphate labeling / Amjad Javed ... [et al.] -- Immunofluorescence analysis using epitope-tagged proteins: in vitro system / Amjad Javed ... [et al.] -- Analysis of in vivo gene expression using epitope-tagged proteins / Amjad Javed ... [et al.] -- Chromatin immunoprecipitation / Amjad Javed ... [et al.] -- , Protein/deoxyribonucleic acid interactions linked to gene expression: electrophoretic mobility shift assay / Amjad Javed ... [et al.] -- Protein/deoxyribonucleic acid interactions linked to gene expression: DNase I digestion / Amjad Javed ... [et al.] -- Protein/deoxyribonucleic acid interactions linked to gene expression: ligation-mediated polymerase chain reaction / Amjad Javed ... [et al.] -- Assays for cyclin-dependent kinase inhibitors / Adrian M. Senderowicz and Tyler Lahusen -- Protein degradation via the proteosome / Henry Hoff, Hong Zhang, and Christian Sell -- , The transformed phenotype / Henry Hoff ... [et al.] -- A morphologic approach to detect apoptosis based on electron microscopy / Martyn K. White, Timothy G. Schneider, and Caterina Cinti -- Detection of apoptotic deoxyribonucleic acid break by in situ / Nick Translation ... [et al.] -- Induction of deoxyribonucleic acid damage by alkylating agents / Salvatore Cortellino ... [et al.] -- Induction of deoxyribonucleic acid damage by - irradiation / Salvatore Cortellino, David P. Turner and Alfonso Bellacosa -- Ultraviolet irradiation of cells / David P. Turner, Anthony T. Yeung, and Alfonso Bellacosa -- Transient production of retroviral- and lentiviral-based vectors for the transduction of mammalian cells / Tiziana Tonini ... [et al.] -- , Determination of functional viral titer by drug-resistance -- Colony assay, expression of green fluorescent protein, and ʹ-galactoside staining / Tiziana Tonini ... [et al.] -- Retroviral and lentiviral vector titration by the analysis of the activity of viral reverse transcriptase / Tiziana Tonini ... [et al.] -- Single and double colloidal gold labeling in postembedding immunoelectron microscopy / Nicoletta Zini ... [et al.] -- Multifluorescence labeling and colocalization analyses / Massimo Riccio ... [et al.].

Giordano, Antonio, , Romano, Gaetano,

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