3350

edited by Wei Xiao

2nd ed

Humana Press

c2006

xi, 392 p. ill.

Methods in molecular biology

Includes bibliographical references and index , Basic investigations in saccharomyces cerevisiae / Brendan P.G. Curran and Virginia Bugeja -- Isolation of nucleic acids / Michelle Hanna and Wei Xiao -- Purification of yeast peroxisomes / Ben Distal and Astrid Kragt -- Isolation of yeast plasma membranes / Barry Panaretou and Peter Piper -- Isolation of yeast mitochondria / Chris Meisinger, Nikolaus Pfanner, and Kaye N. Truscott -- Extraction of yeast lipids / Roger Schneiter and Gunther Daum -- Two-dimensional gel electrophoresis of total yeast proteins / Helian Boucherie and Christelle Monribot-Espagne -- Pulsed field gel electrophoresis of budding yeast chromosomes / Laura Maringele and David Lydall -- Analysis of yeast lipids / Roger Schneiter and Gunther Daum -- Yeast fluorescence microscopy / Jiri Hasek -- , Intracellular expression of recombinant antibody fluorescent protein fusions for localization of target antigens in schizosaccharomyces pombe / Michelle A. Alting-Mees ... [et al.] -- Yeast transformed by the LiAc/SS carrier DNA/PEG method / R. Daniel Gietz and Robin A. Woods -- Mutagenesis / Leslie Barbour, Michelle Hanna, and Wei Xiao -- Gene disruption in the budding yeast saccharomyces cerevisiae / Johannes H. Hegemann, Ulrich Guldener, and Gabriele J. Kohler -- Inducible degron and its application to creating conditional mutants / R. Jurgen Dohmen -- Synthetic lethal screen / Leslie Barbour and Wei Xiao -- Synthetic genetic array analysis in saccharomyces cerevisiae / Amy Hin Yan Tong and Charles Boone -- Two-dimensional agarose gel analysis of DNA replication intermediates / Alain T. Dandjinou ... [et al.] -- Chromatin assembly in a crude fraction from yeast cells / Karen M. Robinson and Michael C. Schultz -- Chromatin immunoprecipitation to study protein/DNA interactions in budding yeast / Elena Ezhkova and William P. Tansey -- , Isolation of yeast nuclei and micrococcal nuclease mapping of nucleosome positioning / Zhengjian Zhang and Joseph C. Reese -- Study of transcriptional regulation using a reporter gene assay / Yu Fu and Wei Xiao -- Assessing telomeric phenotypes / Catherine LeBel ... [et al.] -- Controlled expression of recombinant genes and preparation of cell-free extracts in yeast / Zhigang Wang -- Production of heterologous proteins in yeast with the aid of the Hsp150-Carrier / Marja Makarow ... [et al.] -- Yeast two-hybrid system screening / R. Daniel Gietz -- Import of precursor proteins into isolated yeast mitochondria / Nils Wiedemann, Nikolaus Pfanner, and Peter Rehling.

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