3347

edited by Hua Gu, Klaus Rajewsky

Humana Press

c2004

xi, 289 p. ill.

Methods in molecular biology

Includes bibliographical references and index , Characterization of B lymphopoiesis in mouse bone marrow and spleen / Richard R. Hardy and Susan A. Shinton -- Characterization of B-cell maturation in the peripheral immune system / Rita Carsetti -- Identification of B-cell subsets : an exposition of 11-color (Hi-D) FACS methods / James W. Tung ... [et al.] -- Analysis of B-cell life-span and homeostasis / Irmgard Furster -- Differentiation of B lymphocytes from hemotopoietic stem cells / Paulo Vieira and Ana Cumano -- Analysis of lymphocyte development and function using the rag-deficient blastocyst complementation system / Faith M. Young, Carl A. Pinkert, and Andrea Bottaro -- Conditional gene mutagenesis in B-lineage cells / Stefano Casola -- Analysis of the germinal center reaction and in vivo long-lived plasma cells / Kai-Michael Toellner, Mahmood Khan, and Daniel Man-Yuen Sze -- T-cell-dependent immune responses, germinal center development, and the anaysis of V-gene sequences / Claudia Berek -- , Introduction of genes into primary murine splenic B cells using retrovirus vectors / Kuo-I Lin and Kathryn Calame -- Immunoglobulin class switching: in vitro induction and analysis / Sven Kracker and Andreas Radbruch -- In vitro and in vivo assays of B-lymphocyte migrations / Chantal Moratz and John H. Kehrl -- Analysis of antigen-specific B-cell memory directly ex vivo / Louise J. McHeyzer-Williams and Michael G. McHeyzer-Williams -- B-cell signal transduction : tyrosine phosphorylation, kinase activity, and calcium mobilization / Tilman Brummer ... [et al.] -- Isolation of lipid rafts from B lymphocytes / Anu Cherukuri ... [et al.] -- Molecular single-cell PCR analysis of rearranged immunoglobulin genes as a tool to determine the clonal composition of normal and malignant human B cells -- Analysis of B-cell immune tolerance induction using transgenic mice / Helen Ferry and Richard J. Cornall -- Analysis of B-cell signaling using dt40 B cell line / Tomoharu Yasuda and Tadashi Yamamoto.

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