3341

edited by Cam Patterson, Douglas M. Cyr.

Humana Press

c2005

xi, 381 p. ill.

Methods in molecular biology

Includes bibliographical references and index , Small-molecule inhibitors of proteasome activity / Maria Gaczynska and Pawel A. Osmulski -- Purification of E1 and E1-like enzymes / Art Haas -- Assays for RING family ubiquitin ligases / Manabu Furukawa, Paul Andrews, and Yue Xiong -- Ubiquitin chain synthesis / Shahri Raasi and Cecile M. Pickart -- Purification of proteasomes, proteasome subcomplexes, and proteasome associated proteins from budding yeast / David S. Leggett, Michael H. Glickman, and Daniel Finley -- Recognition and processing of misfolded proteins by PA700, the 19S regulatory complex of the 26S proteasome / Chang-Wei Liu ... [et al.] -- Cell-free assay for ubiquitin-independent proteasomal protein degradation / Chaim Kahana and Yuval Reiss -- , Assays of proteasome-dependent cleavage products / Stefan Tenzer and Hansjorg Schild -- Identification of components of protein complexes / Carol E. Parker, Maria R. Warren, and Christoph H. Borchers -- Mass spectroscometric determination of protein ubiqitination / Carol E. Parker ... [et al.] -- Reconstitution of ER associated degradation using yeast membranes and cytosol / Robert J. Lee, Ardythe A. McCracken, and Jeffrey L. Brodsky -- Reticulocyte lysate as a model system to study ER membrane protein degradation / Eric Carlson ... [et al.] -- Deubiquitinating enzyme purifucation, assay inhibitors and characterization / Nathaniel S. Russell and Keith D. Wilkinson -- Measuring ubiquitin conjugation in cells / Edward G. Mimnaugh and Leonard M. Neckers -- Assays for proteasome assembly and maturation / R. Jurgen Dohmen ... [et al.] -- , N-terminal ubiquitination / Aaron Ciechanover -- Quantitating defective ribosome products / Shu-Bing Qian, Jack R. Bennink, and Jonathan W. Yewdell -- ER-associated quality control and degradation: screen for ERAD mutants after EMS mutagenesis / Antje Schaefer and Dieter H. Wolf -- ER-associated quality control and degradation: genome wide screen for ERAD components / Antje Schaefer and Dieter H. Wolf -- Cystic fibrosis transmembrane conducatance regulator as a model substrate to study ER protein quality control in mammalian cells / J. Michael Younger ... [et al.] -- Aggresome formation / Michael J. Corboy, Philip J. Thomas, and W. Christian Wigley -- Detection of sumoylated proteins / Kevin D. Sarge and Roland S. Hilgarth -- Proteasome inhibitors in cancer therapy / Robert Z. Orlowski -- Parkinson disease: assays for the ubiquitin ligase activity of neural parkin / Michael G. Schlossmacher and Hideki Shimura.

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