3334

mechanisms and protocols

edited by Tim Humphrey, Gavin Brooks

Humana Press

c2005

xii, 402 p. ill.

Methods in molecular biology

Includes bibliographical references and index , Cell cycle molecules and mechanisms of the budding and fission yeasts / Tim Humphrey and Amanda Pearce -- The plant cell cycle / John H. Doonan -- The C. elegans cell cycle / Sander van den Heuvel -- Developmental control of growth and cell cycle progression in drosophila / Lisa Swanhart, Jeremy Kupsco, and Robert J. Duronio -- The xenopus cell cycle / Anna Philpott and P. Renee Yew -- The mammalian cell cycle / Jane Vera Harper and Gavin Brooks -- Synchronization of cell populations in G1/S and G2/M phases of the cell cycle / Jane Vera Harper -- Mapping origins of DNA replication in eukaryotes / Susan A. Gerbi -- In situ assay for analyzing the chromatin binding of proteins in fission yeast / Stephen E. Kearsey ... [et al.] -- Application of magnetic beads to purify cells transiently transfected with plasmids encoding short hairpin RNAs / Peter D. Adams -- The cell cycle and virus infection / Steven Emmett ... [et al.] -- Methods for preparation of proteins and protein complexes that regulate the eukaryotic cell cycle for structural studies / Julie Welburn and Jane Endicott -- E2F transcription factors and pRb pocket proteins in cell cycle progression / Ludger Hhauck and Rudiger von Harsdorf -- Forkhead (FOX) transcription factors and the cell cycle / Katrina A. Bicknell -- Measurement of geminin activity in xenopus egg extracts / Thomas J. McGarry -- CDK-activating kinases / Stephane Larochelle and Robert P. Fisher -- Cyclins, cyclin-dependent kinases, and cyclin-dependent kinase inhibitors / Gavin Brooks -- Measurement of wee kinase activity / Paul R. Mueller and Walter F. Leise III -- CDC25 dual-specificity protein phosphatases / Sorab N. Dalal and Melanie Volkening -- Assaying cell cycle checkpoint / Carmela Palermo and Nancy C. Walworth -- Polo-like kinase-1 / Marcel A.T.M. van Vugt and Rene H. Medema -- The Ipl1/aurora kinase family / Claire Ditchfield, Nicholas Keen, and Stephen S. Taylor -- Purification of the Ndc80 kinetochore subcomplex from xenopus eggs / Mark L. McCleland and P. Todd Stukenberg.

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