2004

edited by Alastair D. Reith.

Humana Press

c2001

359 p. ill.

Methods in molecular biology

Includes bibliographical references , Machine generated contents note: Preface v -- Contributors ix -- 1 Protein Kinase-Mediated Signaling Networks: -- Regulation and Functional Characterization -- Alastair D.Reith1 -- 2 Cloning Protein Tyrosine Kinases by Screening cDNA Libraries -- with Antiphosphotyrosine Antibodies -- Lisa D.Chong and Ira O.Daar 21 -- 3 Immunoprecipitation and Western Blotting of Phosphotyrosine- -- Containing Proteins -- Kathleen M.Woods Ignatoski 39 -- 4 Two-Dimensional Phosphoamino Acid Analysis -- Peter Blume-Jensen and Tony Hunter49 -- 5 Two-Dimensional Phosphopeptide Mapping of Receptor -- Tyrosine Kinases -- Jeremy M.Tavare and Tarik Issad67 -- 6 Identification of the Sites of Phosphorylation in Proteins Using High -- Performance Liquid Chromatography and Mass Spectrometry -- A.Grey Craig 87 -- 7 Phosphorylation of Smad Signaling Proteins by Receptor -- Serine/Threonine Kinases -- Serhiy Souchelnytskyi, Lars R6nnstrand, Carl-Henrik Heldin, -- and Peter ten Dijke .107 -- 8 Assays for Mitogen-Activated Protein Kinase (MAPK) Subtypes -- and MAPK Activating Protein Kinase-2 (MAPKAP K-2) Using -- a Common Cell Lysate -- Rozen Le Panse, Navita Rampersaud, -- and Louis C.Mahadevan 121 -- 9 JAK-Mediated Phosphorylation and Activation of STAT Signaling -- Proteins: Analysis by Phosphotyrosine Blotting and EMSA -- Nicola Broughton and Mark S.Burfoot 131 -- 10 Assays for Glycogen Synthase Kinase-3 (GSK-3) -- Darren Cross 147 -- 11 Cyclin-Dependent Kinases and Cyclin-Dependent Kinase Inhibitors: -- Detection Methods and Activity Measurements -- Gavin Brooks161 -- 12 Protein Histidine Kinase -- Harry R.Matthews and Karina Chan 171 -- 13 Cloning and Expression of Recombinant HEXA-HIS Tagged -- ZAP-70 Using the Baculovirus System -- Tracey Purton, Sandra Wilkinson, and Edward J.Murray183 -- 14 Analysis of Protein Kinase Subcellular Localization -- by Visualization of GFP Fusion Proteins -- Ralph A.Zirngibl and Peter Greer 195 -- 15 Detection of Phosphorylation-Dependent Interactions -- by Far-Western Gel Overlay -- Mary Rose Burnham, Regina DeBerry, and Amy H.Bouton209 -- 16 Purification of Tyrosine-Phosphorylated Proteins -- by Immunoaffinity Chromatography and Direct Cloning -- of Their cDNAs from Bacterial Expression Libraries -- Pier Paolo Di Fiore and Francesca Fazioli 221 -- 17 Identification of Receptor Tyrosine Kinase (RTK) Substrates -- by the Cloning of Receptor Targets (CORT) Strategy -- Roger J.Daly239 -- 18 Identification of Receptor Tyrosine Kinase Associating Proteins -- Using the Yeast Two-Hybrid System -- Sally A.Prigent 251 -- 19 cDNA Expression Cloning and Characterization of Phosphorylation -- Dependent Protein Interactors Using the Yeast Tribrid System -- Christoph Volpers, Manuel Lubinus, Mark A.Osborne, -- and Jarema P.Kochan271 -- 20 Analysis of SH2 Domain-Phosphopeptide Interactions -- by Isothermal Titration Calorimetry and Surface -- Plasmon Resonance -- George Panayotou and John Ladbury295 -- 21 Cloning and Characterization of RTK Ligands Using Receptor- -- Alkaline Phosphatase Fusion Proteins -- Hwai-Jong Cheng and John G.Flanagan 313 -- 22 Isolation and Characterization of "Orphan-RTK" Ligands Using -- an Integrated Biosensor Approach -- Martin Lackmann 335 -- Index 361.

Reith, Alastair D

572.

76

P967

0896037002