مقايسه اثر دو شيوه تمرين ورزشي منتخب روي بيان mir-204 ، mir-181 و نشانگرهاي متابوليكي كودكان مبتلا به ديابت نوع1

Comparison of Two selec‎ted Exercises Training Protocols on Expression of mir-204, mir-181, an‎d Metabolic Biomarkers in Type 1 Diabetic Children

فيزيولوژي ورزش

Background an‎d Purpose: The incidence of metabolic disorders, particularly type 1 diabetes, has risen in recent years, yet there is a scarcity of information regarding effective exercise interventions for managing this condition. Currently, microRNAs are being recognized as valuable clinical instruments for the identification an‎d prevention of non-communicable diseases. The primary objective of this study was to examine the impact of two distinct training modalities—resistance an‎d aerobic exercise—on the expression levels of microRNA-181 an‎d microRNA-204, as well as various metabolic markers in children diagnosed with type 1 diabetes. Methods: This investigation employed a quasi-experimental design featuring a pre-test an‎d post-test framework, conducted in a real-world environment. Eighteen adolescents, comprising both boys an‎d girls aged 10 to 14 years with type 1 diabetes, were ran‎domly assigned to two groups: one engaging in resistance training (n=9; weight: 51.5±15.7 kg, height: 154.5±12.6cm, body mass index: 21.13±4.4 kg/m2) an‎d the other in aerobic training (n=9; weight: 50.9±14.7 kg, height: 154.5±12.6 cm, body mass index: 20.8±3.9 kg/m2). Each group participated in three training sessions per week over an 8-week period. MicroRNA-181 an‎d microRNA-204 were extracted from blood serum using real-time PCR, while insulin, HbA1c, blood sugar an‎d lipid profile levels were quantified via ELISA method. Intra-group comparisons were performed using paired t-tests, an‎d inter-group comparisons were analyzed through the analysis of covariance (ANCOVA) satistic (P≥0.05). Results: The statistical eva‎luation revealed a significant between group difference at the levels of microRNA-181 (1.6% resistance vs. 2.2% aerobic; p=0.324), but changes in microRNA-204 level was not significant (3.46% resistance vs. 2.54% aerobic; p=0.242). Furthermore, alterations in fasting blood glucose levels (resistance 19.34% compared to aerobic 10.58%; p=0.012), insulin concentrations (resistance 79.41% vs. aerobic 45.83%; p=0.985), an‎d HbA1c levels (resistance training -29.85% vs. 30.39%; p=0.001) were not statistically significant. Also, significant differences were not detected in cardiorespiratory endurance level (resistance training 2.93% compared to aerobic 1.65%; p=0.004), nor in the body fat percentage among participants in the various groups (resistance training 2.43% vs. aerobic 3.38%; p=0.259). Conclusion: Both resistance an‎d aerobic exercise modalities appear to yield significant improvements in the assessed parameters. The efficacy of selec‎ted resistance training during adolescence is comparable to that of aerobic exercise, indicating that these training approaches may serve as suitable interventions for children an‎d adolescents diagnosed with type 2 diabetes.

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